Journal of Plant Tissue Culture
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Population Structure and Regeneration Status of Elaeocarpus venustus Bedd. In Agasthiyamalai Biosphere Reserve, Western Ghats, India
S. Saravanan and K. Muthuchelian
India has been identified as one of the top 12 mega-diversity countries of the world. We studied population density, spatial distribution, seedling survival and regeneration potential of E. venustus in past three years. The density of E. venustus was higher in Upper Kodayar (83 individuals) followed by Muthukuzhivoyal (45 individuals) and least was recorded in Kakachi (27 individuals). The age structure of E. venustus clearly indicates that discontinuous population structure and regeneration potential was also very poor. The seed density was high in Upper Kodayar (10 seeds/m2) followed by Muthukuzhivoyal (9 seeds/m2) and least was recorded in Kakachi (7 seeds/m2). Nearly about 40% of the seeds were damaged by invasive beetle. Seedling mortality was high in Kakkachi for 72 % and followed by 62 % in Upper Kodayar, and 56 % in Muthukuzhivoyal.
Keywords: Agasthiyamalai, Endangered, Elaeocaprus venustus, Regeneration potential, Seedling survival, Population structure
In Vitro Propagation of Rain Lily (Zyphyranthes citrine L.)
L. John Peter Arulanandam S. Ghanthikumar and T. Arunkumar
A standard protocol was established for the mass propagation of Zyphyranthes citrine – an important ornamental plant through sterilized bulb scales of Z.citrine, that were cultured on Murashige and Skoog (MS) media supplemented with 0.5-2.5 mg/l of BAP and 0.1-0.5 mg/l of NAA. Maximum percentage (80%) Shoot induction was observed on MS media supplemented with 1.0 mg/l of BAP and 0.2 mg/l of NAA. The in vitro shoots were sub-cultured on MS media augmented with 0.2-1.6mg/l of IBA alone for root induction. Maximum percentage (84%) cluster of roots formation was observed on MS medium augmented with 1.0 mg/l of IBA alone. The well-developed plantlets were subsequently hardened to green house conditions.
Key words: Zyphyranthes citrine, Bulb, MS medium, Micropropagation
Current Volume No. 5(1) March,2016