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Gayathri Publishers is a non profit company in India. The company have published 8 Journals in print and online, and open access, They are covering vast research areas of biological Sciences, Chemical sciences and Medical Sciences.   We have published quality of research articles and open source a lot of articles, to help in the young scholars, scientists and professors. All the free journals are Open Access Journals including Clinical, Medical, and Chemistry, Education, Pharmaceutical, Life Sciences, Environmental, Engineering and Management journals. It can be associated with any academic or scientific databases for greater visibility of the published articles. This open access format is one of the important models of publishing which has been widely appreciated, supported than the traditional toll access journals.

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ISSN   2320 –7825  (Print)

ISSN   2320 –7833  (Online)

Applied Biology and Biotechnology

An International Journal

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 Volume No. 5(1)                              March,2017

Current Volume



Feed additives of herbal powder of Cynodon dactylon and Cassia auriculata treatment of Aeromonas hydrophila infection of Channa punctatus (Bloch1973)


G. Seetha lakshmi and R. Arthi Manju




The present study has been carried out the haematological parameters such as; RBCs, WBCs haemoglobin content and micronucleus test were studied using medicinal plants like Cynodon dactylon and Cassia auriculata on different concentrations (C. dactylon;2g and 3gms Cassia auriculata 2gms and 3gms mixture of C. dactylon and C. auriculata (1:1) 2g, 3g) of formulated diet against 0.3 ml of CFU/ml 105 cells of Aeromonas hydrophila introduced in the Channa punctatus. 2gms of mixture of plants powder formulated diet showed maximum WBCs, RBCs and haemoglobin content than the control and other experimental groups. 2gms of mixture of plants powder formulated diet treated fishes found to be more efficient compared with other experimental and control group. However, the fish showed significant (p<0.05) increased when compared with control groups. A high index of micronucleus formation in C. auriculata plant group B1(2g) due to the clastogenic effect of A. hydrophila were observed while its frequency drastically reduced in mixture plant groups like C1 (2g) and C2 (3g) due to its anti clastogenic effect. Antimutagenic potential of such test plant may be established as potent herbal drug against A. hydrophila.


Keywords: Channa punctatus, Cynodon dactylon, Cassia auriculata, Aeromonas hydrophila