Journal of Plant Tissue Culture
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Applied Biology and Biotechnology
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Volume No. 5(1) March,2017
Feed additives of herbal powder of Cynodon dactylon and Cassia auriculata treatment of Aeromonas hydrophila infection of Channa punctatus (Bloch1973)
G. Seetha lakshmi and R. Arthi Manju
The present study has been carried out the haematological parameters such as; RBCs, WBCs haemoglobin content and micronucleus test were studied using medicinal plants like Cynodon dactylon and Cassia auriculata on different concentrations (C. dactylon;2g and 3gms Cassia auriculata 2gms and 3gms mixture of C. dactylon and C. auriculata (1:1) 2g, 3g) of formulated diet against 0.3 ml of CFU/ml 105 cells of Aeromonas hydrophila introduced in the Channa punctatus. 2gms of mixture of plants powder formulated diet showed maximum WBCs, RBCs and haemoglobin content than the control and other experimental groups. 2gms of mixture of plants powder formulated diet treated fishes found to be more efficient compared with other experimental and control group. However, the fish showed significant (p<0.05) increased when compared with control groups. A high index of micronucleus formation in C. auriculata plant group B1(2g) due to the clastogenic effect of A. hydrophila were observed while its frequency drastically reduced in mixture plant groups like C1 (2g) and C2 (3g) due to its anti clastogenic effect. Antimutagenic potential of such test plant may be established as potent herbal drug against A. hydrophila.
Keywords: Channa punctatus, Cynodon dactylon, Cassia auriculata, Aeromonas hydrophila